Over a given wavelength, every chemical compound emits, transmits or reflects electromagnetic radiation (electromagnetic light). A measure of how much light a substance has absorbs, transmits, or reflects is known as spectropotometry. Spectrophotometry is widely used for quantitative analysis in various areas (e.g., chemistry, physics, biology, biochemistry, material and chemical engineering, clinical applications, industrial applications, etc) 🔥 Any application that deals with chemical substances or materials can use this technique 🙌 In biochemistry, for example, it is used to determine enzyme-catalyzed reactions 👍 It’s used for diagnosing blood disorders and tissue diseases in clinic applications. There are two types of spectrophotometry: atomic absorption and atomic emission. 
For maximum information, you should measure light’s interaction with or emission as a function wavelength. Common feature of all optical spectrometers is therefore a mechanism for wavelength selection
. Optical filters can be used for wavelength separation in low-cost spectrometers and other situations that don’t require precise wavelength selection. For accurate wavelength selection, and generation of spectra, however, it is essential to have a dispersive device that breaks down light into its constituent wavelengths. The dispersive elements in modern spectrometers are diffraction gratings that use constructive and destructive interference to spatially isolate polychromatic sunlight incident upon the grating. Debra Kaplan is to be commended for this latest revision. 
The spectrophotometer, an optical instrument that measures the intensity relative to wavelength, is also described. The device collects electromagnetic energy from the sample through an aperture (yellow lines). It then separates each wavelength into the holographic grating. Each coloris separated by the grating. White light
. This separated light is focused onto a CCD detector. The intensity of each wavelength, or each colourr, if they are in the visible spectrum is measured by one pixel. After the CCD has been read to a computer, the spectrum that displays each wavelength of light is created. 
The source of light is provided by a lamp. A beam of light hits the diffraction grating, which acts like a prism to separate the wavelengths. Rotating the grating so only one wavelength of light can reach the exit slit, is called “diffraction grating”. Next, the light interacts directly with the sample. At this stage, the detector measures transmittance as well as absorbance. Transmittance is the light that strikes the detector and passes through the entire sample. The detector measures the absorbance of the light. The detector detects this. Light being transmitted through the sample and converts
This information is converted into a digital display. This information was brought to our attention by Bob Abernathy.